Recombinant CD43 抗体
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- 抗原 See all CD43 (SPN) 抗体
- CD43 (SPN) (Sialophorin (SPN))
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD43 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of MAb Bra7G is clearly different from that of MAb rSPN/839, called b as opposed to a for rSPN/839. 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human CD43 protein
- 克隆位点
- RSPN-839
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product SPN Primary Antibody
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anti-Sialophorin (SPN) antibody
SPN 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal DF-T1 unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC (p), IP, Func 宿主: 小鼠 Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) (AA 276-400) antibodySPN 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC, IF, StM 宿主: 兔 Monoclonal SPN-1766R unconjugated Recombinant Antibody
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal rSPN-839 unconjugated Recombinant Antibody
anti-Sialophorin (SPN) antibodySPN 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sialophorin (SPN) (AA 1-400) antibodySPN 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Sialophorin (SPN) antibody (FITC)SPN 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-59 FITC
anti-Sialophorin (SPN) antibody (Biotin)SPN 适用: 人 FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-59 Biotin
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K562 cells. Paracortex in a tonsil or a reactive lymph node.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- CD43 (SPN) (Sialophorin (SPN))
- 别名
- SPN (SPN 产品)
- 别名
- CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody
- 背景
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Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN,CD43 (T-Cell Marker)
Cellular localisation: Cell Surface - 分子量
- 95, 115, or 135kDa
- 基因ID
- 6693, 632188
- UniProt
- P16150
- 途径
- Regulation of Leukocyte Mediated Immunity
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