Spastin 抗体
Quick Overview for Spastin 抗体 (ABIN6938422)
抗原
See all Spastin (SPAST) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs.
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交叉反应 （详细）
- Human. Mouse. Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human Spastin protein.
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亚型
- IgG2a kappa
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anti-Spastin (SPAST) (AA 200-304) antibody
SPAST 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F5 unconjugated
anti-Spastin (SPAST) (AA 551-616) antibodySPAST 适用: 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Spastin (SPAST) antibodySPAST 适用: 小鼠, 大鼠, 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Spastin (SPAST) (AA 200-304) antibodySPAST 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated
anti-Spastin (SPAST) antibodySPAST 适用: 小鼠, 大鼠, 人 WB, ELISA, IHC, IF, Coat, StM 宿主: 小鼠 Monoclonal Sp 3G11-1 unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Rat brain extract.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Spastin (SPAST)
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别名
- SPAST
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背景
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ADPSP, FSP2, Spast, Spatin, SPG4,Spastin
Cellular localisation: Cell Membrane and Secreted. -
分子量
- 52kDa
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基因ID
- 6683, 468091
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途径
- Microtubule Dynamics, M Phase, Regulation of Cell Size
抗原
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