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s100a4 抗体 (AA 1-200)

This anti-s100a4 antibody is a 小鼠 单克隆 antibody detecting s100a4 in WB, IHC, IF, FACS 和 IHC (f). Suitable for 人.
产品编号 ABIN6938334
发货至: 中国
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北京 101111
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Room 801-803
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Quick Overview for s100a4 抗体 (AA 1-200) (ABIN6938334)

抗原

See all s100a4 (S100A4) 抗体
s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

适用

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宿主

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小鼠

克隆类型

  • 93
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单克隆

标记

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This s100a4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

S100A4-1481
  • 抗原表位

    • 28
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    • 1
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    AA 1-200

    特异性

    S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

    交叉反应 (详细)

    Human. Mouse.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: PANC-1, HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

    别名

    S100A4

    背景

    S100A4, S100 calcium-binding protein A4, Calvasculin, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein,S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)
    Cellular localisation: Cytoplasmic and nuclear

    分子量

    10-12kDa

    基因ID

    6275, 654444

    UniProt

    P26447

    途径

    S100 Proteins
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