RBP4 抗体
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- 抗原 See all RBP4 抗体
- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This RBP4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Recognizes a protein of 21 kDa-25 kDa, identified as retinol binding protein (RBP). Its epitope localizes between aa 74-182 of human RBP. This MAb recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin). RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.
- 交叉反应 (详细)
- Human. Monkey. Goat. Rabbit. Rat. Mouse.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Retinol binding protein Purified from human plasma
- 克隆位点
- SPM442
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product RBP4 Primary Antibody
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- 应用备注
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Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Liver or placenta.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
- 别名
- RBP (RBP4 产品)
- 背景
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Cellular retinol-binding protein I, CRBP1, CRBP2, RBP1, RBP2, RBP4, RBPC, Retinol binding protein 1, Retinol binding protein 1 cellular, Retinol binding protein 2 cellular, Retinol binding protein 4 plasma,Retinol Binding Protein (RBP)
Cellular localisation: Cytoplasmic - 分子量
- 21-25kDa
- 基因ID
- 5947, 5948, 5950
- UniProt
- P02753, P09455, P50120
- 途径
- Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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