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Proliferation Marker 抗体

适用: 人 IHC, WB, IF, StM 宿主: 小鼠 Monoclonal JC1 unconjugated
产品编号 ABIN6938249
发货至: 中国
  • 抗原
    Proliferation Marker
    适用
    • 3
    • 1
    • 1
    宿主
    • 3
    小鼠
    克隆类型
    • 3
    单克隆
    标记
    • 3
    非结合性
    应用范围
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Staining Methods (StM)
    纯化方法
    Purified by Protein A/G
    免疫原
    Pokeweed-stimulated lymphocytes
    克隆位点
    JC1
    亚型
    IgG2a kappa
  • 应用备注

    Positive Control: HeLa cells (IF). Human lymph node (IHC).

    Known Application: Western Blot (0.5-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 缓冲液
    10 mM PBS without BSA and without Azide.
    储存液
    Azide free
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
    有效期
    24 months
  • 抗原
    Proliferation Marker
    背景
    JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
    分子量
    212kDa and 123kDa
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