Proliferation Marker 抗体
Quick Overview for Proliferation Marker 抗体 (ABIN6938249)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified by Protein A/G
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免疫原
- Pokeweed-stimulated lymphocytes
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亚型
- IgG2a kappa
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应用备注
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Positive Control: HeLa cells (IF). Human lymph node (IHC).
Known Application: Western Blot (0.5-2 μg/mL), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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缓冲液
- 10 mM PBS without BSA and without Azide.
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储存液
- Azide free
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储存条件
- 4 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
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有效期
- 24 months
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- Proliferation Marker
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背景
- JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
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分子量
- 212kDa and 123kDa
抗原
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