PIP 抗体 (AA 41-146)
Quick Overview for PIP 抗体 (AA 41-146) (ABIN6938187)
抗原
See all PIP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 41-146
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特异性
- It recognizes a protein of 15 kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50 % of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human GCDFP-15 protein fragment (around aa 41-146) (exact sequence is proprietary)
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亚型
- IgG2a kappa
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 cells. Breast or Pancreas.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- PIP (Prolactin-Induced Protein (PIP))
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别名
- PIP
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背景
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BRST-2, GCDFP-15, gp17, GPIP4, Gross cystic disease fluid protein 15, Prolactin-induced protein (PIP), Prolactin-inducible protein (PIP), Secretory actin-binding protein (SABP),GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker)
Cellular localisation: Cytoplasmic (Golgi bodies) and Secreted -
分子量
- 15kDa
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基因ID
- 5304, 99949
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UniProt
- P12273
抗原
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