PAX5 抗体 (AA 235-382)
Quick Overview for PAX5 抗体 (AA 235-382) (ABIN6938120)
抗原
See all PAX5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 235-382
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特异性
- The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 21,000, full-length human proteins. The PAX5 gene is a member of thepaired box (PAX)family of transcription factors. The central feature of this gene family is a novel, highly conservedDNA-binding domain, known as thepaired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute toneoplastic transformation. The PAX5 gene encodes theB-celllineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development andspermatogenesis.
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交叉反应 (详细)
- Human. Dog
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- A recombinant fragment (around aa 235-382) of human PAX5 protein (exact sequence is proprietary)
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亚型
- IgG1 kappa
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Daudi or Ramos cells. Human tonsil, spleen, lymph node or colon carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- PAX5 (Paired Box 5 (PAX5))
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别名
- PAX5
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背景
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B cell lineage specific activator, BSAP, EBB-1, KLP, Paired box 5,PAX5 / BSAP (Early B-Cell Marker)
Cellular localisation: Nuclear -
分子量
- 42kDa
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基因ID
- 5079, 654464
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UniProt
- Q02548
抗原
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