Nuclear Antigen (Pan-Nuclear Marker) 抗体
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北京 101111
Quick Overview for Nuclear Antigen (Pan-Nuclear Marker) 抗体 (ABIN6938094)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Nuclear Antigen (Pan-Nuclear Marker) Antibody
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特异性
- This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
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无交叉反应
- 猪
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Nuclei of HL60 cells
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亚型
- IgG1, kappa
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anti-Nuclear Antigen (Pan-Nuclear Marker) antibody
适用: 人, 小鼠, 大鼠 IHC, FACS, IF 宿主: 小鼠 Monoclonal NM106 unconjugated
anti-Nuclear Antigen (Pan-Nuclear Marker) antibody适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Monoclonal NM2984R unconjugated Recombinant Antibody
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应用备注
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Positive Control: All human cells. Tonsil.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Nuclear Antigen (Pan-Nuclear Marker)
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背景
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Nuclear Antigen
All human cells. Tonsil
抗原
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