NPM1 抗体 (AA 185-287)
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- 抗原 See all NPM1 抗体
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
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抗原表位
- AA 185-287
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NPM1 antibody is un-conjugated
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应用范围
- ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Recognizes a 33 kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)
- 克隆位点
- NPM1-3285
- 亚型
- IgG2c kappa
- Top Product
- Discover our top product NPM1 Primary Antibody
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anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 1-294) antibody
NPM1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B2 unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 250-298), (C-Term) antibodyNPM1 适用: 人 WB, IF, IHC (p), IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 20-154) antibodyNPM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 212-292) antibodyNPM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibodyNPM1 适用: 人 WB, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal NPM1-3286 unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (pThr199) antibodyNPM1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibodyNPM1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rNPM1-1901 unconjugated Recombinant Antibody
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 185-287) antibodyNPM1 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal NPM1-1902 unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (C-Term) antibodyVerified NPM1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (AA 7-33) antibodyNPM1 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Known_Application: Immunofluorescence (1-2 μg/mL), ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K562, MCF-7, HeLa or A431 cells. Skin, Colon.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
- 别名
- NPM1 (NPM1 产品)
- 别名
- B23 antibody, NPM antibody, NO38 antibody, Npm antibody, B23NP antibody, npm4 antibody, npm antibody, CG7917 antibody, CRP1 antibody, Crp1 antibody, Dmel\\CG7917 antibody, dNLP antibody, dNLP-S antibody, p22 antibody, npm1 antibody, sb:cb487 antibody, nucleophosmin 1 antibody, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog antibody, nucleophosmin 1b antibody, Nucleophosmin antibody, nucleophosmin antibody, nucleophosmin (nucleolar phosphoprotein B23, numatrin) antibody, Nucleoplasmin antibody, nucleophosmin 1a antibody, NPM1 antibody, Npm1 antibody, npm1.L antibody, npm1b antibody, npm antibody, Nlp antibody, npm1a antibody
- 背景
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NO38, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin (nucleolar phosphoprotein B23 numatrin), Nucleophosmin, Nucleophosmin/nucleoplasmin family member 1, Numatrin,Nucleophosmin (Acute Myeloid Leukemia Marker)
Cellular localisation: Nuclear and Cytoplasmic - 分子量
- 33kDa
- 基因ID
- 4869, 557550
- UniProt
- P06748
- 途径
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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