NME2 抗体
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北京 101111
Quick Overview for NME2 抗体 (ABIN6938079)
抗原
See all NME2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- NME2 / nm23-H2 / NDPK-B (Suppressor of Metastasis) Antibody
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特异性
- The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
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交叉反应 (详细)
- Human.
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纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant full-length human NME2 protein
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亚型
- IgG2a, kappa
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应用备注
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Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: PC3, A549, HeLa, Jurkat cells. Ubiquitously expressed in all tissues.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- NME2 (Nucleoside diphosphate kinase B (NME2))
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别名
- NME2
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背景
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C myc purine binding transcription factor PUF, C-myc purine-binding transcription factor PUF, epididymis secretory sperm binding protein Li 155an, HEL-S-155an, Histidine protein kinase NDKB, NDP kinase B, NDPKB, nm23-H2, NM23B, NME/NM23 nucleoside diphosphate kinase 2, nme2, Nucleoside diphosphate kinase B,
A549, HeLa, Jurkat cells. Ubiquitously expressed in all tissues., PC3
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分子量
- 17kDa
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基因ID
- 4831, 463456
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UniProt
- P22392
抗原
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