NME2 抗体
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北京 101111
Quick Overview for NME2 抗体 (ABIN6938079)
抗原
See all NME2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human NME2 protein
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亚型
- IgG2a kappa
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anti-Nucleoside diphosphate kinase B (NME2) (AA 51-152) antibody
NME2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D3 unconjugated
anti-Nucleoside diphosphate kinase B (NME2) (AA 137-152), (C-Term) antibodyNME2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nucleoside diphosphate kinase B (NME2) (AA 8-149) antibodyNME2 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Nucleoside diphosphate kinase B (NME2) (AA 1-152) antibodyNME2 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 4B7-3F12 unconjugated
anti-Nucleoside diphosphate kinase B (NME2) (AA 1-152) antibodyNME2 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 4G7A8 unconjugated
anti-Nucleoside diphosphate kinase B (NME2) (AA 137-152) antibodyNME2 适用: 人, 小鼠, 大鼠 WB, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nucleoside diphosphate kinase B (NME2) (AA 1-152) antibodyNME2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nucleoside diphosphate kinase B (NME2) antibodyNME2 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal CPTC-NME2-2 unconjugated
anti-Nucleoside diphosphate kinase B (NME2) antibodyKD Validated NME2 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB3115 unconjugated Recombinant Antibody
anti-Nucleoside diphosphate kinase B (NME2) (AA 1-152) antibodyNME2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A7 unconjugated
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应用备注
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Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: PC3, A549, HeLa, Jurkat cells. Ubiquitously expressed in all tissues.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- NME2 (Nucleoside diphosphate kinase B (NME2))
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别名
- NME2
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背景
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C myc purine binding transcription factor PUF, C-myc purine-binding transcription factor PUF, epididymis secretory sperm binding protein Li 155an, HEL-S-155an, Histidine protein kinase NDKB, NDP kinase B, NDPKB, nm23-H2, NM23B, NME/NM23 nucleoside diphosphate kinase 2, nme2, Nucleoside diphosphate kinase B,NME2 / nm23-H2 / NDPK-B (Suppressor of Metastasis)
Cellular localisation: Cytoplasmic and Nuclear -
分子量
- 17kDa
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基因ID
- 4831, 463456
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UniProt
- P22392
抗原
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