NEFH & NEFL 抗体
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北京 101111
Quick Overview for NEFH & NEFL 抗体 (ABIN6938037)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb reacts with a 200 kDa and 68 kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
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交叉反应 （详细）
- Human. Mouse. Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Human NF-H from isolated brain cells
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亚型
- IgG1 kappa
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anti-NEFH & NEFL antibody
适用: 人, 大鼠, 小鼠, Pig, 小鸡 FACS, IHC, StM 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-NEFH & NEFL antibody适用: 人, 大鼠, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal 2F11 unconjugated
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: IMR-32 cells. Cerebellum or Neuroblastoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- NEFH & NEFL
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背景
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NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200. 68 kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68 kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68,Neurofilament (H+L) (Neuronal Marker)
Cellular localisation: Cytoplasmic -
分子量
- 200kDa & 68kDa
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基因ID
- 4744, 198760
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UniProt
- P12036
抗原
-
