MYOD1 抗体 (AA 180-189)
Quick Overview for MYOD1 抗体 (AA 180-189) (ABIN6937997)
抗原
See all MYOD1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 180-189
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特异性
- Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.
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交叉反应 （详细）
- Human. Mouse. Rat. Chicken.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.
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亚型
- IgG1 kappa
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anti-Myogenic Differentiation 1 (MYOD1) (AA 150-250) antibody
MYOD1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 51-150) antibodyMYOD1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) antibodyMYOD1 适用: 人 IHC, StM 宿主: 兔 Monoclonal MYOD1-2075R unconjugated Recombinant Antibody
anti-Myogenic Differentiation 1 (MYOD1) antibodyMYOD1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rMYD712 unconjugated Recombinant Antibody
anti-Myogenic Differentiation 1 (MYOD1) (AA 1-320) antibodyMYOD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) antibodyMYOD1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 211-320) antibodyMYOD1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 1-320) antibodyMYOD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 211-320) antibodyMYOD1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Myogenic Differentiation 1 (MYOD1) (AA 211-240) antibodyMYOD1 适用: 人 WB, IF, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Rhabdomyosarcoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- MYOD1 (Myogenic Differentiation 1 (MYOD1))
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别名
- MYOD1
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背景
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BHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM,MyoD1 (Rhabdomyosarcoma Marker)
Cellular localisation: Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation. -
分子量
- 45kDa
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基因ID
- 4654, 181768
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UniProt
- P15172
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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