Recombinant Moesin 抗体
Quick Overview for Recombinant Moesin 抗体 (ABIN6937915)
抗原
See all Moesin (MSN) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin.
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交叉反应 (详细)
- Human. Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human Moesin protein
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亚型
- IgG1 kappa
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HT-29, Jurkat, CH3LC or HUVEC cells. Uterus, Placenta, Tonsil, Melanoma, Testicular Carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Moesin (MSN)
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别名
- MSN
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背景
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Membrane-organizing extension spike protein, Moesin/anaplastic lymphoma kinase fusion protein, MSN/ALK fusion,Moesin
Cellular localisation: Cytoplasmic & Cell Surface -
分子量
- 78kDa
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基因ID
- 4478, 87752
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UniProt
- P26038
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途径
- Asymmetric Protein Localization
抗原
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