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Recombinant KRT8 抗体

KRT8 适用: 人 WB, IHC (f), ISt 宿主: 兔 Monoclonal KRT8-2174R unconjugated Recombinant Antibody
产品编号 ABIN6937756
发货至: 中国
  • 抗原 See all KRT8 抗体
    KRT8 (Keratin 8 (KRT8))
    抗体类型
    Recombinant Antibody
    适用
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    宿主
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    克隆类型
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    单克隆
    标记
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    This KRT8 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)
    特异性
    Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast.
    交叉反应 (详细)
    Human. Sheep. Rabbit. Cow. Pig.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Recombinant full-length human KRT8 protein
    克隆位点
    KRT8-2174R
    亚型
    IgG
    Top Product
    Discover our top product KRT8 Primary Antibody
  • 应用备注

    Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Positive_Control: HCT116, MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    KRT8 (Keratin 8 (KRT8))
    别名
    KRT8 (KRT8 产品)
    别名
    CARD2 antibody, CK-8 antibody, CK8 antibody, CYK8 antibody, K2C8 antibody, K8 antibody, KO antibody, AA960620 antibody, AL022697 antibody, AU019895 antibody, Card2 antibody, EndoA antibody, Krt-2.8 antibody, Krt2-8 antibody, CYKER antibody, KRT2-8 antibody, KERATIN8 antibody, ck8 antibody, cyk8 antibody, k2c8 antibody, card2 antibody, krt2-5 antibody, MGC69490 antibody, KRT8 antibody, DreK8 antibody, cb186 antibody, krt2-8 antibody, sb:cb186 antibody, wu:fa20h05 antibody, wu:fa95h10 antibody, wu:fb96c06 antibody, zf-K8 antibody, zfk8 antibody, DKFZp468F2127 antibody, keratin 8 antibody, keratin 8 S homeolog antibody, KRT8 antibody, Krt8 antibody, krt8.S antibody, krt8 antibody
    背景
    CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, K2C8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8,Cytokeratin 8 (KRT8)
    Cellular localisation: Cytoplasmic
    分子量
    52.5kDa
    基因ID
    3856, 533782, 708445
    UniProt
    P05787
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