Keratin 77 抗体
Quick Overview for Keratin 77 抗体 (ABIN6937737)
抗原
See all Keratin 77 (KRT77) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
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交叉反应 （详细）
- Human. Monkey. Cow. Dog. Rabbit. Mouse. Rat. Chicken. Turtle.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Human epidermal keratin
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亚型
- IgG1 kappa
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anti-Keratin 77 (KRT77) antibody
KRT77 适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal KRTL-1077 unconjugated
anti-Keratin 77 (KRT77) antibodyKRT77 适用: 人, 大鼠, Cow, 犬, 小鼠, 兔, 小鸡, 猴, Turtle IHC, WB, IF, FACS, StM 宿主: 小鼠 Monoclonal AE-1 unconjugated
anti-Keratin 77 (KRT77) antibodyKRT77 适用: 人 IHC, StM 宿主: 兔 Monoclonal KRTL-1577R unconjugated Recombinant Antibody
anti-Keratin 77 (KRT77) (Internal Region) antibodyKRT77 适用: 人, 大鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Keratin 77 (KRT77) (AA 1-578) antibodyKRT77 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Skin or squamous cell carcinoma (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Keratin 77 (KRT77)
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别名
- KRT77
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背景
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K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39,Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)
Cellular localisation: Cytoplasmic -
分子量
- 40-56.5kDa
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基因ID
- 374454, 334989
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UniProt
- Q7Z794
抗原
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