Recombinant Cytokeratin 2 抗体
Quick Overview for Recombinant Cytokeratin 2 抗体 (ABIN6937734)
抗原
See all Cytokeratin 2 (KRT76) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
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交叉反应 （详细）
- Human. Mouse. Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Solubilized keratin extract from human stratum corneum
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亚型
- IgG
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anti-Cytokeratin-2P (KRT76) antibody
KRT76 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 34BE12 unconjugated
anti-Cytokeratin-2P (KRT76) antibodyKRT76 适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal KRTH-1076 unconjugated
anti-Cytokeratin-2P (KRT76) (AA 173-283) antibodyKRT76 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cytokeratin-2P (KRT76) antibodyKRT76 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, StM 宿主: 兔 Monoclonal KRTH-2147R unconjugated Recombinant Antibody
anti-Cytokeratin-2P (KRT76) antibodyKRT76 适用: 人 IHC, StM 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
anti-Cytokeratin-2P (KRT76) antibodyKRT76 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cytokeratin-2P (KRT76) antibodyKRT76 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal rKRTH-2148 unconjugated Recombinant Antibody
anti-Cytokeratin-2P (KRT76) (AA 1-638) antibodyKRT76 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7, PC12 cells, Skin, Prostate carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))
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别名
- KRT76
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背景
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KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Basic (Type II or HMW)
Cellular localisation: Cytoplasmic -
分子量
- 50-67kDa
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基因ID
- 51350, 654392
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UniProt
- Q01546
抗原
-
