Cytokeratin 2 抗体
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北京 101111
Quick Overview for Cytokeratin 2 抗体 (ABIN6937731)
抗原
See all Cytokeratin 2 (KRT76) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Cytokeratin, Basic (Type II or HMW) Antibody
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特异性
- This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.
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交叉反应 (详细)
- Human.
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纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction
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亚型
- IgG1, kappa
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: PC12 cells. Tonsil, Skin, Prostate, Esophagus, Ectocervix or Squamous carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))
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别名
- KRT76
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背景
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KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
Ectocervix or Squamous carcinoma., Esophagus, PC12 cells. Tonsil, prostate, Skin
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分子量
- 50-67kDa
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基因ID
- 51350, 654392
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UniProt
- Q01546
抗原
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