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Cytokeratin 2 抗体

This anti-Cytokeratin 2 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 2 in WB, IF, FACS 和 IHC (f). Suitable for 人.
产品编号 ABIN6937731
发货至: 中国

Quick Overview for Cytokeratin 2 抗体 (ABIN6937731)

抗原

See all Cytokeratin 2 (KRT76) 抗体
Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

DE-SQ
  • 特异性

    This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: PC12 cells. Tonsil, Skin, Prostate, Esophagus, Ectocervix or Squamous carcinoma.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

    别名

    KRT76

    背景

    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Basic (Type II or HMW)
    Cellular localisation: Cytoplasmic

    分子量

    50-67kDa

    基因ID

    51350, 654392

    UniProt

    Q01546
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