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KRT4 抗体 (AA 181-292)

This anti-KRT4 antibody is a 小鼠 单克隆 antibody detecting KRT4 in FACS, IF 和 IHC (f). Suitable for 人.
产品编号 ABIN6937710
发货至: 中国

Quick Overview for KRT4 抗体 (AA 181-292) (ABIN6937710)

抗原

See all KRT4 抗体
KRT4 (Keratin 4 (KRT4))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This KRT4 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

KRT4-2804
  • 抗原表位

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    AA 181-292

    特异性

    Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13). It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment (around aa 181-292) of human KRT4 protein (exact sequence is proprietary)

    亚型

    IgG1 kappa
  • 应用备注

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HeLa, A549 or A431 cells. Skin, Cervix or Esophagus.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    KRT4 (Keratin 4 (KRT4))

    别名

    KRT4

    背景

    CK4, CYK4, Cytokeratin 4, Keratin 4, Keratin type II cytoskeletal 4, KRT4,Cytokeratin 4 (KRT4)
    Cellular localisation: Cytoplasmic and Cell Surface

    分子量

    67kDa

    基因ID

    3851, 654610, 706527

    UniProt

    P19013
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