KRT4 抗体 (AA 181-292)
Quick Overview for KRT4 抗体 (AA 181-292) (ABIN6937710)
抗原
See all KRT4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 181-292
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特异性
- Cytokeratin 4 (KRT4) is an intermediate filament protein associated with Cytokeratin 13 (KRT13). It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium of esophagus, cornea, anus, larynx, pharynx and tongue. Decreased expression of KRT4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from that of thyroid origin.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment (around aa 181-292) of human KRT4 protein (exact sequence is proprietary)
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亚型
- IgG1 kappa
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, A549 or A431 cells. Skin, Cervix or Esophagus.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- KRT4 (Keratin 4 (KRT4))
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别名
- KRT4
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背景
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CK4, CYK4, Cytokeratin 4, Keratin 4, Keratin type II cytoskeletal 4, KRT4,Cytokeratin 4 (KRT4)
Cellular localisation: Cytoplasmic and Cell Surface -
分子量
- 67kDa
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基因ID
- 3851, 654610, 706527
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UniProt
- P19013
抗原
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