Recombinant Cytokeratin 19 抗体
Quick Overview for Recombinant Cytokeratin 19 抗体 (ABIN6937690)
抗原
See all Cytokeratin 19 (KRT19) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- This Ab reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40 kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50 % -60 % of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
-
交叉反应 (详细)
- Human. Mouse.
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Recombinant human full-length KRT19 protein
-
亚型
- IgG
-
-
-
-
应用备注
-
Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Hep-G2, MCF-7, HeLa cells, Breast Cancer.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
-
别名
- KRT19
-
背景
-
K19, k1cs, Keratin 19 Keratin Type i 40kD, krt19,Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker)
Cellular localisation: Cytoplasmic -
分子量
- 40kDa
-
基因ID
- 3880, 654568
-
UniProt
- P08727
抗原
-