IgL 抗体
Quick Overview for IgL 抗体 (ABIN6937549)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Purified IgG from human serum
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亚型
- IgG1 kappa
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anti-IgL antibody
适用: 人 IHC, FACS, IF, WB, StM 宿主: 小鼠 Monoclonal HP6054 unconjugated
anti-IgL antibody适用: 人 IHC, StM 宿主: 兔 Monoclonal LLC-3778R unconjugated Recombinant Antibody
anti-IgL (AA 1-232) antibody适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-IgL antibody (CF®488A)适用: 人 FACS, IF, T 宿主: 小鼠 Monoclonal HP6054 CF®488A
anti-IgL antibody适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rLLC-3777 unconjugated Recombinant Antibody
anti-IgL antibody适用: 人 IHC, FACS, IF, WB, StM 宿主: 小鼠 Monoclonal N10-2 unconjugated
anti-IgL antibody适用: 人 Coat, ELISA 宿主: 小鼠 Monoclonal ICO-106 unconjugated
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应用备注
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Known_Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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