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Recombinant ICAM-3/CD50 抗体

ICAM3 适用: 人 IHC (f), Bind 宿主: 兔 Monoclonal ICAM3-2873R unconjugated Recombinant Antibody
产品编号 ABIN6937471
发货至: 中国
  • 抗原 See all ICAM-3/CD50 (ICAM3) 抗体
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    抗体类型
    Recombinant Antibody
    适用
    • 109
    • 3
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    宿主
    • 67
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    克隆类型
    • 69
    • 40
    单克隆
    标记
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    This ICAM-3/CD50 antibody is un-conjugated
    应用范围
    • 60
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    Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Binding Studies (Bind)
    特异性
    Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
    交叉反应 (详细)
    Human.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Recombinant full-length human ICAM3 protein
    克隆位点
    ICAM3-2873R
    亚型
    IgG
    Top Product
    Discover our top product ICAM3 Primary Antibody
  • 应用备注

    Known_Application: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order Ab without Azide), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HL-60 or THP-1 cells. Lymph node and tonsil.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    别名
    ICAM3 (ICAM3 产品)
    别名
    CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody
    背景
    CD50, CDW 50, CDw50, ICAM 3, ICAM-3, ICAM-R, ICAM3, ICAM3_HUMAN, ICAMR, Intercellular adhesion molecule 3,CD50 / ICAM3
    Cellular localisation: Membrane.
    分子量
    110-160kDa
    基因ID
    3385, 654563
    UniProt
    P32942
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