Recombinant ICAM-3/CD50 抗体
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- 抗原 See all ICAM-3/CD50 (ICAM3) 抗体
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This ICAM-3/CD50 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Binding Studies (Bind)
- 特异性
- Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human ICAM3 protein
- 克隆位点
- ICAM3-2873R
- 亚型
- IgG
- Top Product
- Discover our top product ICAM3 Primary Antibody
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- 应用备注
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Known_Application: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order Ab without Azide), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HL-60 or THP-1 cells. Lymph node and tonsil.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
- 别名
- ICAM3 (ICAM3 产品)
- 别名
- CD50 antibody, CDW50 antibody, ICAM-R antibody, ICAM-3 antibody, ICAM3 antibody, intercellular adhesion molecule 3 antibody, ICAM3 antibody, Icam3 antibody
- 背景
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CD50, CDW 50, CDw50, ICAM 3, ICAM-3, ICAM-R, ICAM3, ICAM3_HUMAN, ICAMR, Intercellular adhesion molecule 3,CD50 / ICAM3
Cellular localisation: Membrane. - 分子量
- 110-160kDa
- 基因ID
- 3385, 654563
- UniProt
- P32942
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