ICAM-3/CD50 抗体
Quick Overview for ICAM-3/CD50 抗体 (ABIN6937465)
抗原
See all ICAM-3/CD50 (ICAM3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Stimulated human leukocytes
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亚型
- IgG2b kappa
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anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody
ICAM3 适用: 人 FACS, IHC (p), Func 宿主: 小鼠 Monoclonal MEM-04 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibodyICAM3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-171 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-203) antibodyICAM3 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2D11D1 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 200-547) antibodyICAM3 适用: 人 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-547) antibodyICAM3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F8 unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (Biotin)ICAM3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-171 Biotin
anti-Intercellular Adhesion Molecule 3 (ICAM3) antibody (PE)ICAM3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-171 PE
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 46-197) antibodyICAM3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 30-130) antibodyICAM3 适用: 人 ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Intercellular Adhesion Molecule 3 (ICAM3) (AA 259-491) antibodyICAM3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order Ab without Azide),Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HL-60 or THP-1 cells. Lymph node and tonsil.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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别名
- ICAM3
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背景
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ICAMR, Intercellular adhesion molecule 3 (ICAM3),CD50 / ICAM3
Cellular localisation: Cell surface -
分子量
- 110-160kDa
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基因ID
- 3385, 354563
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UniProt
- P32942
抗原
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