CD235a/GYPA 抗体
Quick Overview for CD235a/GYPA 抗体 (ABIN6937353)
抗原
See all CD235a/GYPA (GYPA) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a sialoglycoprotein of 39 kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus. Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant human full-length GYPA protein
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亚型
- IgG1 kappa
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Erythrocytes in any human tissues. K562 cell lysate.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
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别名
- GYPA
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背景
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Blood group--MN locus, GPA, GPErik, GpMiIII, GPSAT, GYPA, MN sialoglycoprotein, MNS, PAS2, Sialoglycoprotein alpha,Glycophorin A / CD235a (Erythrocyte Marker)
Cellular localisation: Cell surface -
分子量
- 39kDa
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基因ID
- 2993, 2994, 434973
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UniProt
- P02724
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途径
- Maintenance of Protein Location
抗原
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