Geminin 抗体
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北京 101111
Quick Overview for Geminin 抗体 (ABIN6937327)
抗原
See all Geminin (GMNN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Geminin / DNA Replication Inhibitor Antibody
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特异性
- Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.
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交叉反应 （详细）
- Human.
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纯化方法
- 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Recombinant human full-lengthprotein
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亚型
- IgG1
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anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 110-209) antibody
GMNN 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1A8 unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 1-206) antibodyGMNN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) antibodyGMNN 适用: 人 IHC, IF 宿主: 小鼠 Monoclonal CPTC-GMNN-1 unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 110-142) antibodyGMNN 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 110-209) antibodyGMNN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B10 unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 1-209) antibodyGMNN 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) antibodyKD Validated GMNN 适用: 人 WB 宿主: 兔 Monoclonal 25GB3475 unconjugated Recombinant Antibody
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 96-123) antibodyGMNN 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB20074 unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 21-196) antibodyGMNN 适用: 人, 小鼠 WB, IHC, IF, ELISA, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 1-209) antibodyGMNN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HEK293, HeLa or MCF-7 cells. Human tonsil or triple-negative breast cancer.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))
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别名
- GMMN
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背景
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DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, GMNN, RP3 369A17.3
HEK293, HeLa or MCF-7 cells. Human tonsil, testis or triple-negative breast cancer tissue.
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分子量
- 35kDa
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基因ID
- 51053, 234896
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UniProt
- O75496
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途径
- EGFR Signaling Pathway, DNA Replication
抗原
-
