GFAP 抗体
Quick Overview for GFAP 抗体 (ABIN6937306)
抗原
See all GFAP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
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交叉反应 （详细）
- Human. Mouse. Rat. Cow. Pig. Rabbit. Chicken.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- GFAP isolated from pig spinal cord
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亚型
- IgG1 kappa
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anti-Glial Fibrillary Acidic Protein (GFAP) (AA 51-150) antibody
GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibodyGFAP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 93-432) antibodyGFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 253-354) antibodyGFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 1-430) antibodyGFAP 适用: 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 292-432) antibodyGFAP 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 小鼠 Monoclonal 1C91F1 unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (C-Term) antibodyVerified GFAP 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (pSer38) antibodyGFAP 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) antibodyGFAP 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Glial Fibrillary Acidic Protein (GFAP) (AA 108-211) antibodyGFAP 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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别名
- GFAP
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背景
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Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP),GFAP (Astrocyte & Neural Stem Cell Marker)
Cellular localisation: Cytoplasmic -
分子量
- ~50kDa
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基因ID
- 2670, 514227
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UniProt
- P14136
抗原
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