Recombinant CNN1 抗体
Quick Overview for Recombinant CNN1 抗体 (ABIN6937008)
抗原
See all CNN1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
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交叉反应 （详细）
- Human. Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human CNN1 protein
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亚型
- IgG1 kappa
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anti-Calponin 1 (CNN1) (AA 1-268) antibody
CNN1 适用: 人 IHC, WB, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Calponin 1 (CNN1) (AA 201-297) antibodyCNN1 适用: 人, 大鼠, 小鼠 FACS, WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Calponin 1 (CNN1) antibodyCNN1 适用: 人, 大鼠 FACS, IF, IHC, StM 宿主: 兔 Monoclonal CNN1-1408R unconjugated Recombinant Antibody
anti-Calponin 1 (CNN1) antibodyCNN1 适用: 人, 大鼠 FACS, IF, IHC, WB, StM 宿主: 小鼠 Monoclonal CALP unconjugated
anti-Calponin 1 (CNN1) (AA 16-165) antibodyCNN1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1H5B5 unconjugated
anti-Calponin 1 (CNN1) antibodyCNN1 适用: 人, 大鼠 FACS, IHC, WB, StM 宿主: 小鼠 Monoclonal rCNN1-832 unconjugated Recombinant Antibody
anti-Calponin 1 (CNN1) (AA 10-39), (N-Term) antibodyCNN1 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB19472 unconjugated
anti-Calponin 1 (CNN1) (AA 1-297) antibodyCNN1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F5-1H4 unconjugated
anti-Calponin 1 (CNN1) (C-Term) antibodyCNN1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-455R unconjugated Recombinant Antibody
anti-Calponin 1 (CNN1) antibodyCNN1 适用: 人 IHC, WB, ELISA 宿主: 兔 Monoclonal 2H1 unconjugated Recombinant Antibody
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: K562 cells, Myoepithelial cells in breast ducts.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CNN1 (Calponin 1 (CNN1))
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别名
- CNN1
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背景
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Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC,Calponin-1 (Smooth Muscle Marker)
Cellular localisation: Cytoplasmic -
分子量
- 34kDa
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基因ID
- 1264, 465929
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UniProt
- P51911
抗原
-
