CNN1 抗体
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- 抗原 See all CNN1 抗体
- CNN1 (Calponin 1 (CNN1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CNN1 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
- 交叉反应 (详细)
- Human. Rat.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Crude human uterus extract
- 克隆位点
- CALP
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CNN1 Primary Antibody
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- 应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: K562 cells, Myoepithelial cells in breast ducts.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- CNN1 (Calponin 1 (CNN1))
- 别名
- CNN1 (CNN1 产品)
- 别名
- CN antibody, CnnI antibody, SMCC antibody, Sm-Calp antibody, XclpH3 antibody, clpH3 antibody, cnn2 antibody, calponin 1 antibody, calponin 1, basic, smooth muscle L homeolog antibody, cnn1 antibody, Cnn1 antibody, CNN1 antibody, cnn1.L antibody
- 背景
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Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC,Calponin-1 (Smooth Muscle Marker)
Cellular localisation: Cytoplasmic - 分子量
- 34kDa
- 基因ID
- 1264, 465929
- UniProt
- P51911
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