CLTA/CLTB 抗体 (N-Term)
Quick Overview for CLTA/CLTB 抗体 (N-Term) (ABIN6937002)
抗原
See all CLTA/CLTB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Purified recombinant N-terminal fragment of human Clathrin Light Chain
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亚型
- IgG2b kappa
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anti-Clathrin, Light Chain A/B (CLTA/CLTB) (N-Term) antibody
CLTA/CLTB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CLC-1421 unconjugated
anti-Clathrin, Light Chain A/B (CLTA/CLTB) (N-Term) antibodyCLTA/CLTB 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal SPM174 unconjugated
anti-Clathrin, Light Chain A/B (CLTA/CLTB) (C-Term) antibodyCLTA/CLTB 适用: 人, 小鼠, 大鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Placenta or Prostate Carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))
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别名
- CLTA and CLTB
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背景
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Clathrin light chain A, Clathrin light chain B, Clathrin light chain LCA, Clathrin light chain LCB, Clathrin light polypeptide A, Clathrin light polypeptide B, Clathrin, light polypeptide (Lca), Clathrin, light polypeptide (Lcb), CLTA, CLTB,Clathrin, Light Chain
Cellular localisation: Cytoplasmic -
分子量
- 31-44kDa
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基因ID
- 1211, 1212, 484241
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UniProt
- P09496, P09497
抗原
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