CHD4 抗体 (C-Term)
Quick Overview for CHD4 抗体 (C-Term) (ABIN6936972)
抗原
See all CHD4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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特异性
- CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.
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交叉反应 （详细）
- Human. Mouse.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.
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亚型
- IgG2a kappa
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anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) antibody
CHD4 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1632-1730) antibodyCHD4 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4H4 unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1520-1690) antibodyCHD4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (C-Term) antibodyCHD4 适用: Synthetic WB, ELISA, IF, FACS, Coat, IHC (f) 宿主: 小鼠 Monoclonal 3F2-4 unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1-219) antibodyCHD4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (N-Term) antibodyCHD4 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 1-194) antibodyCHD4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3D3E9 unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) (AA 71-120) antibodyCHD4 适用: 人, 小鼠, 大鼠, Pig, Cow, 犬, 豚鼠, 马, 兔, Bat, 小鸡, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) antibodyCHD4 适用: 人, 小鼠, 大鼠, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) antibodyCHD4 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal 3F2-4 unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))
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别名
- CHD4
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背景
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ATP dependent helicase CHD4, Chromodomain helicase DNA binding protein 4, Mi 2 autoantigen 218 kDa protein, Mi 2b, Mi2 beta,CHD4
Cellular localisation: Nucleus and cytoplasm. -
分子量
- 238kDa
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基因ID
- 1108, 162233
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UniProt
- Q14839
抗原
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