CGA (HCG-Alpha) & CGB (HCG-Beta) 抗体
适用: 人
IHC (f)
宿主: 小鼠
Monoclonal
HCGab-52
unconjugated
-
- 抗原
- CGA (HCG-Alpha) & CGB (HCG-Beta)
- 适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- This MAb is a very special because it reacts ONLY with the intact-HCG and not with either free alpha- or free beta-chain of HCG. HCG is a glycoprotein and is composed of two non-identical, non-covalently linked polypeptide chains designated as the subunit is identical to that of thyroid stimulating hormone (TSH) follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.
- 交叉反应 （详细）
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Purified hCG protein
- 克隆位点
- HCGab-52
- 亚型
- IgG1 kappa
-
anti-CGA (HCG-Alpha) & CGB (HCG-Beta) antibody
适用: 人 IHC, StM 宿主: 小鼠 Monoclonal HCGab-52 unconjugated
-
- 应用备注
-
Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: JAR or TT cells. Placenta.
- 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
-
- 抗原
- CGA (HCG-Alpha) & CGB (HCG-Beta)
- 背景
-
CG-alpha, CGA, Chorionic Gonadotrophin Alpha, Follicle Stimulating Hormone Alpha, Follitropin Alpha, FSH-alpha, FSHA, GPH Alpha, GPHA1, LHA, LH-alpha, Luteinizing Hormone Alpha, Lutropin Alpha, Thyroid Stimulating Hormone Alpha, Thyrotropin Alpha, TSHA, CG-beta, CGB3, CGB5, CGB7, CGB8, Choriogonadotropin Subunit beta, hCGB,HCG Holo (Pregnancy & Choriocarcinoma Marker)
Cellular localisation: Cytoplasmic, Secreted - 分子量
- ~35kDa (intact hCG)
- 基因ID
- 1081, 1082, 172944
- UniProt
- P01215, P01233
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