CDK1 抗体 (C-Term)
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Quick Overview for CDK1 抗体 (C-Term) (ABIN6936870)
抗原
See all CDK1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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特异性
- Recognizes a 34 kDa protein (cdk1), identified as p34cdc2 (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2). It shows no cross reaction with cdk2 p32. It supports the kinase activity. p34cdc2 plays a crucial role during cell division and is most active during mitosis. It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by a tyrosine kinase. This MAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.
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交叉反应 （详细）
- Human. Mouse. Rat. Guinea Pig. Woodchuck. Xenopus. Chicken.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli
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亚型
- IgG2a kappa
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anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 59-182) antibody
CDK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 208-258) antibodyCDK1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 1 (CDK1) (pTyr15) antibodyCDK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 211-297) antibodyCDK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G10 unconjugated
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) antibodyCDK1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A4-1A9 unconjugated
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) antibodyCDK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 211-297) antibodyCDK1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 8F1 unconjugated
anti-Cyclin-Dependent Kinase 1 (CDK1) antibodyCDK1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 66-297) antibodyCDK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase 1 (CDK1) (AA 201-297) antibodyCDK1 适用: 人, 小鼠, 大鼠, 犬, 绵羊 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Inhibits activation of p34cdc2 kinase by cyclins,Immunofluorescence (1-2 μg/mL), Immunoprecipitation, Kinase Assay, Western Blot, ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells. Human tonsil (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
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别名
- CDC2
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背景
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Cdc2, CDC28A, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cyclin-dependent kinase 1, p34 Protein Kinase, P34CDC2,Cdk1 / p34cdc2 Serine-Threonine Kinase
Cellular localisation: Nuclear and Cytoplasmic -
分子量
- 34kDa
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基因ID
- 983, 334562
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UniProt
- P06493
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途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
抗原
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