CD53 抗体
Quick Overview for CD53 抗体 (ABIN6936755)
抗原
See all CD53 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop V, Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.
-
交叉反应 （详细）
- Human.
-
纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
-
免疫原
- Human Sezary cells
-
亚型
- IgG2b kappa
-
-
anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7A8D3 unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1H2C7 unconjugated
anti-CD53 (CD53) antibody (PE)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 PE
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-53 unconjugated
anti-CD53 (CD53) (AA 100-180) antibodyCD53 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) (Internal Region) antibodyCD53 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IF, IP, IHC 宿主: 兔 Chimeric HD77 unconjugated Recombinant Antibody
anti-CD53 (CD53) antibody (FITC)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 FITC
anti-CD53 (CD53) (AA 65-160) antibodyCD53 适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) (AA 1-219) antibodyCD53 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
-
-
应用备注
-
Known_Application: Functional Studies (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
Positive_Control: Daudi, Raji and Jurkat cells. Human leukocytes. Human tonsil and lymph node.
-
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
-
储存液
- Azide free
-
储存条件
- -20 °C,-80 °C
-
储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
-
有效期
- 24 months
-
-
- CD53
-
别名
- CD53
-
背景
-
CD53 glycoprotein, CD53 tetraspan antigen, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, Tetraspanin-25 (Tspan-25), TSPAN25,CD53 (TSPAN25)
Cellular localisation: Cell Surface -
分子量
- 33-55kDa
-
基因ID
- 963, 443057
-
UniProt
- P19397
抗原
-
