CD47 抗体 (AA 18-135)
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Quick Overview for CD47 抗体 (AA 18-135) (ABIN6936741)
抗原
See all CD47 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 18-135
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原理
- CD47 / IAP (Integrin Associated Protein) Antibody
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特异性
- This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
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交叉反应 （详细）
- Human.
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纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)
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亚型
- IgG2c, kappa
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anti-CD47 (CD47) (AA 20-140) antibody
CD47 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) antibodyCD47 适用: 人 FACS, WB, IF, Func 宿主: 小鼠 Monoclonal B6H12-2 unconjugated
anti-CD47 (CD47) (AA 18-135) antibodyCD47 适用: 人 FACS, IHC, IF, ELISA 宿主: 小鼠 Monoclonal CD47-3019 unconjugated
anti-CD47 (CD47) (AA 241-323) antibodyCD47 适用: 小鼠, 大鼠 FACS, WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) (AA 18-219) antibodyCD47 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD47 (CD47) antibody (FITC)CD47 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-122 FITC
anti-CD47 (CD47) (AA 18-135) antibodyCD47 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal CD47-2937 unconjugated
anti-CD47 (CD47) antibodyCD47 适用: 人, Pig, Non-Human Primate FACS, WB, IHC (fro) 宿主: 小鼠 Monoclonal MEM-122 unconjugated
anti-CD47 (CD47) antibodyCD47 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Monoclonal 4G11 unconjugated Recombinant Antibody
anti-CD47 (CD47) (AA 19-142) antibodyCD47 适用: 人 WB, IHC, ICC 宿主: 兔 Monoclonal BLR131H unconjugated Recombinant Antibody
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- CD47
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别名
- CD47
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背景
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Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen
MCF-7, Jurkat, THP-1 or U-87 cells. Human prostate.
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分子量
- ~50kDa
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基因ID
- 961, 446414
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UniProt
- Q08722
抗原
-
