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CD19 抗体 (AA 96-281)

This anti-CD19 antibody is a 小鼠 单克隆 antibody detecting CD19 in FACS, IHC, ELISA, Coat 和 IHC (f). Suitable for 人.
产品编号 ABIN6936615
发货至: 中国

Quick Overview for CD19 抗体 (AA 96-281) (ABIN6936615)

抗原

See all CD19 抗体
CD19 (CD19 Molecule (CD19))

适用

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小鼠

克隆类型

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单克隆

标记

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This CD19 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

CD19-3117
  • 抗原表位

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    AA 96-281

    特异性

    CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.

    交叉反应 (详细)

    Human.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant fragment of human CD19 protein (around aa96-281) (exact sequence is proprietary)

    亚型

    IgG2b kappa
  • 应用备注

    Known_Application: ELISA (For coating, order antibody without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: Raji cells (FACS). Tonsil, Lymph Node or Spleen (IHC).

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    CD19 (CD19 Molecule (CD19))

    别名

    CD19

    背景

    B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, CVID3, Leu-12, T-cell surface antigen Leu-12,CD19 (B-Lymphocyte Marker)
    Cellular localisation: Cell Surface

    分子量

    95kDa

    基因ID

    930, 652262

    UniProt

    P15391

    途径

    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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