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Recombinant Caldesmon 抗体

CALD1 适用: 人 WB, IHC (f) 宿主: 兔 Monoclonal CALD1-1424R unconjugated Recombinant Antibody
产品编号 ABIN6936573
发货至: 中国
  • 抗原 See all Caldesmon (CALD1) 抗体
    Caldesmon (CALD1) (Caldesmon 1 (CALD1))
    抗体类型
    Recombinant Antibody
    适用
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    宿主
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    克隆类型
    • 84
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    单克隆
    标记
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    This Caldesmon antibody is un-conjugated
    应用范围
    • 68
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    Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
    交叉反应 (详细)
    Human and Rat. Others not known.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Recombinant human full-length CALD1 protein
    克隆位点
    CALD1-1424R
    亚型
    IgG
    Top Product
    Discover our top product CALD1 Primary Antibody
  • 应用备注

    Known_Application: Western Blot (1-2 μg/mL), ,Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: Ovary, Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    Caldesmon (CALD1) (Caldesmon 1 (CALD1))
    别名
    CALD1 (CALD1 产品)
    别名
    CDM antibody, H-CAD antibody, HCAD antibody, L-CAD antibody, LCAD antibody, NAG22 antibody, l-Cad antibody, 4833423D12Rik antibody, AI195384 antibody, AV071549 antibody, AW536160 antibody, C920027I18Rik antibody, CALD1 antibody, Caldesmon antibody, caldesmon 1 antibody, CALD1 antibody, Cald1 antibody
    背景
    CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22,Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)
    Cellular localisation: Cytoplasmic
    分子量
    150kDa
    基因ID
    800, 490203
    UniProt
    Q05682
    途径
    Myometrial Relaxation and Contraction
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