CA9 抗体
Quick Overview for CA9 抗体 (ABIN6936554)
抗原
See all CA9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
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交叉反应 （详细）
- Human. Horse.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Microsomal fraction of human renal cortical tissue homogenate
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亚型
- IgG2b kappa
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anti-Carbonic Anhydrase IX (CA9) (AA 37-186) antibody
CA9 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7C4A5 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 59-414) antibodyCA9 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 37-186) antibodyCA9 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 10F7A8 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 52-151) antibodyCA9 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 113-143) antibodyCA9 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 251-350) antibodyCA9 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 9B5 unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人 WB, IP 宿主: 小鼠 Monoclonal 12F10 unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人, 马 WB, IHC, StM 宿主: 小鼠 Monoclonal CA9-781 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 113-143) antibodyCA9 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18383 unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Normal kidney or renal cell carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CA9 (Carbonic Anhydrase IX (CA9))
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别名
- CA9
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背景
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CA-IX, CA9, CAH9_HUMAN, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, P54/58N, pMW1, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250,Renal Cell Carcinoma / gp200 (Carbonic Anhydrase IX)
Cellular localisation: Cell surface and cytoplasmic -
分子量
- 200kDa
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基因ID
- 768, 63287
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UniProt
- Q16790
抗原
-
