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Liver Arginase 抗体 (AA 11-97)

The 小鼠 单克隆 anti-Liver Arginase antibody is suitable to detect Liver Arginase in samples from 人. It has been validated for WB 和 IHC (f).
产品编号 ABIN6936429
发货至: 中国
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Quick Overview for Liver Arginase 抗体 (AA 11-97) (ABIN6936429)

抗原

See all Liver Arginase (ARG1) 抗体
Liver Arginase (ARG1) (Arginase, Liver (ARG1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Liver Arginase antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

ARG1-1125
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    AA 11-97

    特异性

    Recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

    交叉反应 (详细)

    Human. Others not known.

    纯化方法

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    免疫原

    Recombinant human ARG1 protein fragment (around aa11-97) (exact sequence is proprietary)

    亚型

    IgG3 kappa
  • 应用备注

    Known_Application: Western Blotting (1-2 μg/mL),Immunohistology (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: 293Tcells. Hepatocellular Carcinoma (HCC).

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    储存液

    Azide free

    储存条件

    -20 °C,-80 °C

    储存方法

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    有效期

    24 months
  • 抗原

    Liver Arginase (ARG1) (Arginase, Liver (ARG1))

    别名

    ARG1

    背景

    Arginase 1, ARG1, liver-type arginase, type I arginase,Arginase1 (Hepatocellular Carcinoma Marker)
    Cellular localisation: Cytoplasmic

    分子量

    35-38kDa

    基因ID

    383, 440934

    UniProt

    P05089

    途径

    Cellular Response to Molecule of Bacterial Origin
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