alpha Fetoprotein 抗体
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- 抗原 See all alpha Fetoprotein (AFP) 抗体
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This alpha Fetoprotein antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human Alpha feto protein
- 克隆位点
- C2-C3-MBS-12
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product AFP Primary Antibody
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- 应用备注
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Known_Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HepG2 cells. Fetal liver or hepatocellular carcinoma.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- 别名
- AFP (AFP 产品)
- 别名
- FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
- 背景
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Alpha fetoglobulin, FETA, HPAFP,AFP (Alpha Fetoprotein) (Hepatocellular/Germ Cell Tumor Marker)
Cellular localisation: Cytoplasmic - 分子量
- 70kDa
- 基因ID
- 174, 518808
- UniProt
- P02771
- 途径
- C21-Steroid Hormone Metabolic Process
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