ACTA1/ACTA2A/ACTG2 抗体
Quick Overview for ACTA1/ACTA2A/ACTG2 抗体 (ABIN6936352)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
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交叉反应 （详细）
- Human, Rabbit and Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Synthetic peptide common among human muscle actins
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亚型
- IgG1 kappa
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anti-ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth) antibody
适用: 人, 兔, 大鼠 IHC, StM, WB 宿主: 小鼠 Monoclonal MSA-953 unconjugated
anti-ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth) antibody适用: 人, 兔, 大鼠, Cat, 小鸡, 犬, 小鼠 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal HHF35 unconjugated
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应用备注
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Known_Application: Western Blot (0.5-1.0 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Muscle or sarcoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))
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背景
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ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1,Actin, Muscle Specific (Muscle Cell Marker)
Cellular localisation: Cytoplasm. -
分子量
- Multiple
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基因ID
- 58, 59, 70
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UniProt
- P62736, P68032, P68133
抗原
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