ABO 抗体
Quick Overview for ABO 抗体 (ABIN6936339)
抗原
See all ABO 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using 5.000 samples of blood. The MAb HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This MAb is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Mixture of erythrocytes of group B and glyco protein fraction isolated from saliva of secretors with blood group B
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亚型
- IgG1 kappa
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anti-Histo-blood group A transferase (ABO) (AA 54-354) antibody
ABO 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 273-354) antibodyABO 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1B7 unconjugated
anti-Histo-blood group A transferase (ABO) (N-Term) antibodyABO 适用: 人, 小鼠, 大鼠 ELISA, IHC, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 41-68), (N-Term) antibodyABO 适用: 人 WB 宿主: 小鼠 Monoclonal 593CT3-2-2 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 IHC, IF, AGG, H, StM 宿主: 小鼠 Monoclonal HEB-20 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人, 小鼠, 大鼠 IHC, WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) (AA 235-354) antibodyABO 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 IHC, IF, AGG, H, StM 宿主: 小鼠 Monoclonal HE-193 unconjugated
anti-Histo-blood group A transferase (ABO) antibodyABO 适用: 人 IHC, IF, StM 宿主: 小鼠 Monoclonal 19-OLE unconjugated
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应用备注
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Known_Application: Agglutination, Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: KG1 cells. Human colorectal carcinoma tissues.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- ABO (Histo-blood group A transferase (ABO))
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别名
- ABO
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背景
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A transferase, ABO, B transferase, CD173, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT,Blood Group Antigen B (CD173)
Cellular localisation: Cell Surface -
分子量
- Multiple
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基因ID
- 28, 654423
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UniProt
- P16442
抗原
-
