Glypican 3 抗体 (AA 511-580) (CF®555)
Quick Overview for Glypican 3 抗体 (AA 511-580) (CF®555) (ABIN6934330)
抗原
See all Glypican 3 (GPC3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 511-580
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纯化方法
- Purified by Protein A/G
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免疫原
- A recombinant fragment containing amino acids 511-580 of human glypican-3
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亚型
- IgG1 kappa
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应用备注
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Positive Control: 293T cells. Hepatocellular carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 100 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Glypican 3 (GPC3)
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别名
- GPC3
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背景
- Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
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分子量
- 67kDa
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基因ID
- 2719
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UniProt
- P51654
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途径
- Glycosaminoglycan Metabolic Process
抗原
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