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Occludin 抗体 (AA 431-522)

Name: Occludin 抗体 (AA 431-522)
SKU: ABIN687337
Availability: OutOfStock
Rating: 5 (1 reviews)

This anti-Occludin antibody is a 兔多克隆 antibody detecting Occludin in WB, ELISA, FACS, IF (cc), IF (p) 和 IHC (p). Suitable for 人, 小鼠和 Pig. Independently validated for use in Immunofluorescence. This Primary Antibody has been cited in 5+ publications.

Immunofluorescence validation image for anti-Occludin (OCLN) (AA 431-522) antibody (ABIN687337) (Occludin 抗体 (AA 431-522))

Human A549 lysates probed with Rabbit Anti-Occludin Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (Occludin 抗体 (AA 431-522))

HQT regulates epithelial proliferation in the colonic mucosa of mice with DSS-induced acute and chronic colitis. (Occludin 抗体 (AA 431-522))

IHC (p)

Formalin-fixed and mouse kidney tissue labeled with Anti-Occludin Polyclonal Antibody, Unconjugated (ABIN687337) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Occludin 抗体 (AA 431-522))

10 images

(5 references)

(1 validation)

产品编号 ABIN687337

发货至: 中国

Datasheet as PDF

Quick Overview for Occludin 抗体 (AA 431-522) (ABIN687337)

抗原

See all Occludin (OCLN) 抗体

Occludin (OCLN)

适用

人, 小鼠, Pig

宿主

兔

克隆类型

多克隆

标记

This Occludin antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

抗原表位
23

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AA 431-522

交叉反应

人, 小鼠, Pig

预测反应

Rat,Dog,Cow,Horse,Guinea Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Occludin

亚型

IgG
anti-Occludin (OCLN) (AA 263-522) antibody
OCLN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Occludin (OCLN) (C-Term) antibody
OCLN 适用: 人 WB, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Occludin (OCLN) (AA 423-522) antibody
OCLN 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 1G7 unconjugated

anti-Occludin (OCLN) (AA 1-522) antibody
OCLN 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Occludin (OCLN) (AA 1-522) antibody
OCLN 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Occludin (OCLN) (AA 282-415) antibody
OCLN 适用: 人 WB, IHC (f) 宿主: 小鼠 Monoclonal OCLN-2181 unconjugated

anti-Occludin (OCLN) (AA 285-430) antibody
OCLN 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Occludin (OCLN) (C-Term) antibody
OCLN 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal OC-3F10 unconjugated

anti-Occludin (OCLN) (AA 282-415) antibody
OCLN 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-415M unconjugated

anti-Occludin (OCLN) (AA 350-450) antibody
OCLN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029632 (Immunofluorescence)

by

Confocal Imaging Core, Beth Israel Deaconess Medical Center

No.

#029632

日期

2014.03.23

抗原

Lot Number

990698W

Method validated

Immunofluorescence

Positive Control

Mouse stomach tissue

Negative Control

Human fat tissue

Notes

Signal was detected in positive control sample and not in negative control sample.

Validation Images

Figure 1: Immunostaining (green) of mouse stomach tissue (A-C) and human fat tissue (D-F) with anti-OCLN (A, D), isotype control antibody (B, E), or with secondary alone (C, F). Tissues were counterstained with DAPI (blue).
Full Methods
Primary Antibody

Antibody: Occludin (OCLN)
Catalog number: ABIN687337
Lot number: 990698W

Secondary Antibody

Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)

Full Protocol

Paraffin embedded positive and negative control tissue sections were deparaffinized and undergone antigen retrieval using 10 mM Sodium Citrate pH6 in a pressure cooker for 10 min.
The tissue sections were incubated with 1 mg/mL Sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
Tissue sections were rinsed three times in TBS for 5 min each at RT.
Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
Tissue sections were rinsed three times in TBS for 5 min each at RT.
Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen)
IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.

Experimental Notes

No challenges notes
OCLN is recommended to staining of mouse stomach tissue according to this protocol.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Sun, Liu, Xue, Wang: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." in: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, (2015) (PubMed).

Zhao, Qin, Han, Wang, Zhang, Liu: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, (2014) (PubMed).

Pan, Qin, Zhao, Wang, Liu, Che: "Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets." in: International journal of molecular sciences, Vol. 14, Issue 11, pp. 21689-704, (2013) (PubMed).

Zhao, Qin, Sun, Che, Bao, Zhang: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." in: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, (2012) (PubMed).

Gu, Xue, Wei, Zhang, Li: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." in: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, (2011) (PubMed).
抗原

Occludin (OCLN)

别名

Occludin

背景

Synonyms: Ocl, AI53564, Occludin, Ocln
Background: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

基因ID

18260

UniProt

Q61146

途径

Cell-Cell Junction Organization, Hepatitis C