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IL-25 抗体

The 大鼠 单克隆 anti-IL-25 antibody (ABIN6774203) specifically detects IL-25 in ELISA 和 FACS. The antibody is reactive with 小鼠 samples.
产品编号 ABIN6774203
发货至: 中国
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Quick Overview for IL-25 抗体 (ABIN6774203)

抗原

See all IL-25 (IL25) 抗体
IL-25 (IL25) (Interleukin 25 (IL25))

适用

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小鼠

宿主

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大鼠

克隆类型

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单克隆

标记

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This IL-25 antibody is un-conjugated

应用范围

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ELISA, Flow Cytometry (FACS)
  • 特异性

    Recognizes mouse IL-17E. In sandwich ELISAs no cross-reactivity or interference was observed with rhIL-17E, rmIL-17, rmIL-17B, C, D, or F.

    纯化方法

    Protein G purified

    免疫原

    E. coli-derived rmIL-17E.

    Type of Immunogen: Recombinant protein

    亚型

    IgG2b
  • 应用备注

    Approved: ELISA, Flo

    Usage: Suitable for use in ELISA and Flow Cytometry. ELISA (Capture): 2-8 μg/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard. Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    40-50 % glycerol, PBS.

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS, 5 % trehalose.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    IL-25 (IL25) (Interleukin 25 (IL25))

    别名

    IL25 / IL17E

    背景

    Name/Gene ID: IL25
    Family: Interleukin

    Synonyms: IL25, Interleukin 17E, Interleukin 25, Interleukin-25, IL17E, IL-17E, IL-25, Interleukin-17E

    基因ID

    64806

    UniProt

    Q9H293
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