IL-25 抗体
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- 抗原 See all IL-25 (IL25) 抗体
- IL-25 (IL25) (Interleukin 25 (IL25))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-25 antibody is un-conjugated
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应用范围
- ELISA, Flow Cytometry (FACS)
- 特异性
- Recognizes mouse IL-17E. In sandwich ELISAs no cross-reactivity or interference was observed with rhIL-17E, rmIL-17, rmIL-17B, C, D, or F.
- 纯化方法
- Protein G purified
- 免疫原
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E. coli-derived rmIL-17E.
Type of Immunogen: Recombinant protein - 亚型
- IgG2b
- Top Product
- Discover our top product IL25 Primary Antibody
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- 应用备注
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Approved: ELISA, Flo
Usage: Suitable for use in ELISA and Flow Cytometry. ELISA (Capture): 2-8 μg/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard. Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 40-50 % glycerol, PBS.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, 5 % trehalose.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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