IL-25 抗体
Quick Overview for IL-25 抗体 (ABIN6774203)
抗原
See all IL-25 (IL25) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes mouse IL-17E. In sandwich ELISAs no cross-reactivity or interference was observed with rhIL-17E, rmIL-17, rmIL-17B, C, D, or F.
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纯化方法
- Protein G purified
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免疫原
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E. coli-derived rmIL-17E.
Type of Immunogen: Recombinant protein -
亚型
- IgG2b
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应用备注
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Approved: ELISA, Flo
Usage: Suitable for use in ELISA and Flow Cytometry. ELISA (Capture): 2-8 μg/m. Can be used as a capture reagent in a sandwich immunoassay in combination with a biotinylated, mouse IL-17E antibody and recombinant mouse IL-17E, as the standard. Flow Cytometry: For intracellular flow cytometry to detect IL-17E, cells must first be fixed and permeabilized with 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibody may be visualized by adding a secondary developing reagent such as anti-rat IgG conjugated to a fluorochrome. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 40-50 % glycerol, PBS.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- IL-25 (IL25) (Interleukin 25 (IL25))
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别名
- IL25 / IL17E
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背景
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Name/Gene ID: IL25
Family: Interleukin
Synonyms: IL25, Interleukin 17E, Interleukin 25, Interleukin-25, IL17E, IL-17E, IL-25, Interleukin-17E -
基因ID
- 64806
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UniProt
- Q9H293
抗原
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