Sialylated Mucin-Type-O-Glycans 抗体
适用: 人
IHC (p), ELISA, IHC, ICC
宿主: 小鼠
Monoclonal
FW17
unconjugated
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- 抗原
- Sialylated Mucin-Type-O-Glycans
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 特异性
- Sialylated Mucin-Type-O-Glycans.
- 纯化方法
- Purified
- 免疫原
- Purified mucus-glycoproteins from human amniotic fluid.
- 克隆位点
- FW17
- 亚型
- IgM
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- 应用备注
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Approved: ELISA, ICC, IHC, IHC-P
Usage: Tumor and normal tissues were first fixed in 6 % neutral phosphate-buffered formalin and dehydrated in ethanol by standard histological techniques. After embedding all tissues in paraffin, serial microtome tissue sections (5 μm) were used for comparative immunohistological studies. Reactivity of hybridoma tissue culture supernatants was determined using a four-step immunoperoxidase technique. After deparaffinization in xylene, rehydration through graded ethanol, and blocking endogenous peroxidase activity with 2 % H202 in absolute methanol for 30 min, the sections were incubated as follows: (1) normal swine serum (5 % in PBS), (2) MAb in tissue culture supernatant or mouse ascites fluid diluted to 1/100 in PBS, (3) rabbit anti-mouse immunoglobulin (2 % in PBS), (4) swine anti-rabbit immunoglobulin (2 % in PBS), (5) rabbit peroxidase anti-peroxidase (PAP) complex. The slides were treated with 0.2 mg/mL - ' 3-amino-9-ethylcarbazole in 0.02 M sodium acetate buffer containing 0.01 % H202, then counterstained with hematoxylin, and mounted in glycerol jelly. All incubation steps were performed in a moist chamber at 21 °C for 30 min. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
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- 抗原
- Sialylated Mucin-Type-O-Glycans
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