LDLR 抗体 (AA 184-196)
Quick Overview for LDLR 抗体 (AA 184-196) (ABIN233315)
抗原
See all LDLR 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 184-196
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特异性
- Recognizes human LDLR. Does not inhibit binding of LDL. In Western Blot, recognizes the 160kD of LDLR and a 120kD band of the LDLR precursor from fibroblasts, hepatocytes and monocytic cells cultured in the presence of lipoprotein-deficient serum. Sequence not conserved in VLVL Receptor and LRP. Species cross-reactivity: rat. Species sequence Homology: mouse and bovine.
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纯化方法
- Protein A purified
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免疫原
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Synthetic peptide of the complement homology region within the ligand binding site of the LDL receptor extracellular domain. Epitope between aa184-196, the linker region between repeats 4 and 5.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, Flo (1:5), IHC, IHC-Fr (1:10), WB (1:50 - 1:100)
Usage: Suitable for use in ELISA, Western Blot, FACS and Immunohistochemistry. Western Blot: 1:50-1:100 using ECL (non-reducing). FACS: 1:5. Immunohistochemistry: 1:10. Note: It is best to work with partially purified material. In the case of rat (probably also mouse), induce LDLR expression prior to the isolation of culture cells or tissue homogenate. It is always better to use cultured cells. In rat, use estradiol to induce LDLR expression. Positive control: HepG2 cells and liver sections. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 0.5 % BSA.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- LDLR (Low Density Lipoprotein Receptor (LDLR))
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别名
- LDLR / LDL Receptor
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背景
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Name/Gene ID: LDLR
Synonyms: LDLR, FHC, LDL receptor, LDLCQ2 -
基因ID
- 3949
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UniProt
- P01130
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途径
- Hepatitis C, Lipid Metabolism
抗原
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