Fx1a 抗体
Cited in 1 publication.
The 兔 多克隆 anti-Fx1a antibody (ABIN675159) specifically detects Fx1a in IF (cc), IF (p), ELISA, IHC (fro) 和 IHC (p).
The antibody is reactive with 人, 小鼠 和 大鼠 samples.
(1 reference)
产品编号 ABIN675159
发货至:
中国
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Quick Overview for Fx1a 抗体 (ABIN675159)
抗原
Fx1a
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified by Protein A.
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免疫原
- Fx1A protein
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亚型
- IgG
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应用备注
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ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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: "Rapamycin upregulates autophagy by inhibiting the mTOR-ULK1 pathway, resulting in reduced podocyte injury." in: PLoS ONE, Vol. 8, Issue 5, pp. e63799, (2013) (PubMed).
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- Fx1a
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背景
- Optional[synonyms]: Binding of anti-Fx1A to Heymann nephritis antigens (HA) on rat glomerular epithelial cells (GECs) in culture leads to capping and disappearance of antigens from the cell surface. This process may contribute to the formation of glomerular subepithelial immune deposits in vivo. The authors differentially extracted GECs to determine whether HA redistribution is mediated by cytoskeletal components. Observations were made by phase-contrast and immunofluorescence microscopy on primary and passaged GECs in monolayer culture and by spectrofluorimetry on GECs in suspension.
抗原
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