CYR61 抗体 (AA 251-379)
(1 validation)Quick Overview for CYR61 抗体 (AA 251-379) (ABIN674499)
抗原
See all CYR61 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 251-379
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交叉反应
- 人
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预测反应
- Mouse,Rat,Dog,Cow,Sheep,Horse
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human Cyr61
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亚型
- IgG
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anti-Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) (AA 178-381) antibody
CYR61 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) (AA 176-379) antibodyCYR61 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) antibodyCYR61 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) (Center) antibodyCYR61 适用: 人, 大鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) (AA 25-381) antibodyCYR61 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) (AA 143-174) antibodyCYR61 适用: 人 WB 宿主: 兔 Polyclonal RB53929 unconjugated
anti-Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) (AA 47-332) antibodyCYR61 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) antibodyCYR61 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) (AA 25-381) antibodyCYR61 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3C11 unconjugated
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应用备注
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WB 1:300-5000
ELISA 1:500-1000 -
限制
- 仅限研究用
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- by
- Memorial Sloan-Kettering Cancer Center, Parada lab
- No.
- #100003
- 日期
- 2015.10.15
- 抗原
- CYR61
- Lot Number
- Method validated
- Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections)
- Positive Control
- Tumor cell transplanted mouse brain was dissected
- Negative Control
- Notes
- The staining pattern was as expected for Cyr61 as a secreted protein.
- Primary Antibody
- ABIN674499
- Secondary Antibody
- Donkey-anti-Rabbit-Cy3
- Full Protocol
- Tumor cell transplanted mouse brain was dissected, fixed in 4% PFA for overnight, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
- Immunostaining for Cryo Sections:
- Dry slides at 50-55°C for 15-30 min (or RT for 30min)
- Wash three times with PBS 5min (shake gently)
- Block with 10% Normal Donkey Serum (NDS) at RM for 1hour
- Incubate with primary antibody at 4°C overnight (Prepared in 2% NDS)
- Rabbit-anti-CYR61 antibody (ABIN674499) diluted 1:50-1:200
- Wash three times with PBS 5min (shake gently)
- Incubate with secondary antibody together with DAPI at RT for 1h (Prepared in 2% NDS), cover the chamber with foil
- Donkey-anti-Rabbit-Cy3
- DAPI 1:100
- Wash three times with PBS 5min (shake gently)
- Add three drops of water-based Immu-mount
- Cover with 24x60 cover glass
- Imaging
- Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
- Experimental Notes
- ABIN674499 worked within the diution range 1:50 - 1:200
生效 #100003 (Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections))!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IF validation of CYR61 antibody ABIN674499 on mouse brain tumor sections. A. ABIN674499 diluted 1:100 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:100. D. Dilution factor 1:200.]()
IF validation of CYR61 antibody ABIN674499 on mouse brain tumor sections. A. ABIN674499 diluted 1:100 (red), DAPI counterstaining (blue). B. Dilution factor 1:50. C. Dilution factor 1:100. D. Dilution factor 1:200.
Full Methods -
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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- CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))
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别名
- Cyr61
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背景
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Synonyms: CCN1, GIG1, IGFBP10, Protein CYR61, CCN family member 1, Cysteine-rich angiogenic inducer 61, Insulin-like growth factor-binding protein 10, IBP-10, IGF-binding protein 10, IGFBP-10, Protein GIG1, CYR61
Background: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
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基因ID
- 3491
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UniProt
- O00622
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途径
- Positive Regulation of Endopeptidase Activity, Growth Factor Binding
抗原
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