Septin 11 抗体 (N-Term)
Quick Overview for Septin 11 抗体 (N-Term) (ABIN6744459)
抗原
See all Septin 11 (SEPT11) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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品牌
- IHC-plus™
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特异性
- Human SEPT11 / Septin 11
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预测反应
- Percent identity by BLAST analysis: Human, Orangutan, Monkey, Mouse, Rat, Bovine (100%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide from N-Terminus of human SEPT11 (Q9NVA2, NP_060713). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Opossum (85%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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anti-Septin 11 (SEPT11) (AA 237-429) antibody
SEPT11 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Septin 11 (SEPT11) (N-Term) antibodySEPT11 适用: 人, 小鼠, 大鼠, 马, Cow, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Septin 11 (SEPT11) (AA 71-180) antibodySEPT11 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E5 unconjugated
anti-Septin 11 (SEPT11) (N-Term) antibodySEPT11 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Septin 11 (SEPT11) (AA 1-120) antibodySEPT11 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Septin 11 (SEPT11) (N-Term) antibodySEPT11 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Distilled water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS with 2 % sucrose
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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